waters
Xevo QTof MS
9년
주장비
분석
화합물 전처리·분석장비 > 질량분석장비 > 액체크로마토그래피질량분석기
2013-06-10
336,546,000원
기관의뢰
고정형
건별
15,000원
1. It is the most sensitive, exact-mass, quantitative and qualitative benchtop MS/MS system available.
2. The next generation exact mass API MS/MS platform for the Pharmaceutical, Biotechnology and Life Science industries.
3. Ultra performance hybrid tandem Mass spectrometer, it is a mass spectrometer tool for peptide sequencing, protein characterization and metabolite identification.
4. The System is a high-sensitivity detector for off-line and on-line liquid chromatography. Also it has enhanced high-resolution and enables accurate mass measurements to be obtained in both MS and MS-MS modes.
5. High capacity pumping system with fully automated vacuum control, monitoring and protection.
6. To one system not only sample cleanup, separation quickly and easily but also innovative valving for column trapping LC separation.
7. Fully software supported autotuning facilities including ; Automatic calibration routines, HPLC inlet method, API (ESI or APCI in positive and negative ion modes) MS analysis, Data-base search, Peptide de-novo sequencing, Protein identification and Metabolite ID.
1. Hybrid MS/MS system
1) MS1 analyzer : Quadrupole MS analyzer
- Quadrupole with pre-filter and post-filter
- Pre-filter to maximize resolution and eliminate main filter cleaning
- Post-filter to provide optimum transmission, especially at high mass
- Mass range : 20~16,000m/z in non-resolving mode and 20~4,000 in resolving mode
- Analyser parameters used for data acquisition are automatically recorded and appended to the relevant data file
2) COLLISION CELL : The instrument is equipped with a collision cell where the collision energy and gas pressure are under programmable software control. The collision energy and collision gas pressure used during data acquisition are automatically recorded and appended to the relevant data file
3) Dynamic range : Five orders of magnitude
4) MS2 analyzer : Time of flight MS analyzer
- Mass range : 20~100,000m/z
5) Detector
- Ultra-fast electron multiplier and hybrid ADC detector electronics to provide outstanding sensitivity and quantitative performance.
6) Vacuum System
- Differential pumped vacuum system : baking pump, air-cooled turbo molecular pumps.
- Vacuum read backs and system vent/pump cycles are digitally monitored and controlled, to provide total software control and ensure fail-safe operation in the event of power failure.
7) General Specification.
(1) Mass Resolution :
> 22,500 (FWHM) on the (M+6H)6+ isotope cluster from bovine insulin (m/z 956)
(2) MS sensitivity(ESI+)
a) 50 pg/ul of leucine enkephalin (m/z 556 Da) intensity of greater than 1632 counts/sec at > 22,500 FWHM resolution
b) 50 pg/ul of leucine enkephalin (m/z 556 Da) intensity of greater than 3264 counts/sec at > 10,000 FWHM resolution
(3) MS sensitivity(ESI-)
a) 500 pg/ul of raffinose (m/z 503 Da) intensity of greater than 1728 counts/sec at > 22,500 FWHM resolution
b) 500 pg/ul of raffinose (m/z 503 Da) intensity of greater than 3456 counts/sec at > 10,000 FWHM resolution
(4) Acquisition Rate : up to 30 Spectra/sec
(5) Mass Measurement accuracy : better than 1 ppm RMS
(6) Mass Range
- The TOF mass range is m/z 20-100,000
- The Quadrupole mass range is m/z 20-16,000 in non-resolving mode and m/z 20-4,000 in resolving mode
(7) MS/MS sensitivity
- [Glu1]-Fibrinopeptide B solution of 100 fmol/uL at > 22,500 resolution, be greater than 125 counts per second.
2. Sample inlet system
1) API ionization interface
- Tool-free source exchange
- Vacuum isolation valve
- Tool-free access to user serviceable elements
- Plug-and-play probes
- De-clustering cone gas
- Software control of gas flows and heating elements
- Multi-mode source( ESI and APCI) : Multi-mode source capability is allowing both ESI and APCI to be performed, during one experiment, with the same source.
- Enables rapid switching from LC-MS/MS to GC-MS/MS applications on a single instrument
platform. For the analysis of volatile and semi-volatile compounds of low and
intermediate polarity.
2) ESI Ionization probe
- Dual inlet atmospheric pressure ionization source
3. System control and acquisition system
1) Data System
(1) CPU : Intel Core 2 Duo E8400 Processor (3.0 GHz)
(2) Memory : 2GB SDRAM
(3) Internal Hard Disk Drive : 250 GB
(4) Diskette drive : Floppy Disk Drive
(5) DVD Multi-Burner
(6) Networking : Intel PRO 100/1000 Gigabit Ethernet
2) Control software for Hybrid MS/MS system
(1) Optimized Applications : High Throughput applications
(2) Ability to collect nominal or exact mass MS and MS/MS data
(3) Data display
- Reconstructed analogue display within a window on the workstation terminal of the beam transmitted through the instrument which is suitable for tuning purposes.
- Real time chromatogram and spectrum display on the workstation terminal during acquisition
(4) Instrument control and data processing architecture
- All functions relating to instrument control, diagnostic system surveillance and Data acquisition are performed by an embedded PC and a time to digital converter (TDC) or analog digital converter (ADC)
- Post acquisition processing, general data manipulation and networking are carried out by a computer workstation
(5) Instrument control and data processing utilities
a) Tune page acquisitions
- MS acquisition
- MS/MS acquisitions
b) Method editor/sample list acquisitions
- MS acquisition
- MS/MS acquisition
① Data dependant acquisition (DDA) : Describes acquisitions whereby the instrument automatically switches into MS/MS mode during an acquisition based upon data acquired in the MS mode in the previous scan.
② Exact Parent Ion discovery and Exact Neutral loss : Describes acquisitions where, at user-defined intervals, the instrument alternates between low and high-energy conditions.
③ MSE function : Alternates between high-and low collision energies. Enables to acquire all sample information in a single injection. Provides a rapid and accurate snapshot for fragment, precursor and neutral loss information in a data-independent manner. This function should be automatically controlled by software.
c) Tuning Utilities
- Fully software supported instrument tuning via interactive graphics display window allowing control
3) Specifications of LC can be controlled
1) Type : Quaternary Precise, reliable gradient performance, low-pressure mixing
2) Number of Solvents : One to four, in any combination as standard. Up to nine total with optional solvent select valve.
3) Solvent Storage : Solvent tray accommodates up to 4 chromatographic solvents.
4) Solvent Conditioning : Integrated vacuum degassing four chambers. One additional for the Sample Manager with Flow-Through Needle (SM-FTN) wash.
5) Operating Flow rate Range : 0.010 to 2.000 mL/min, in 0.001 mL increments
6) pH Range : 2 ~ 12
7) Total System Delay Volume : <400μL
8) Pump Seal Wash : Equipped with a wash system to flush the rear of the high pressure seal and the plunger.
9) Gradient Profiles : Eleven(11) gradient curves[including linear, step[2], concave(4), and convex(4)].
10) Primary Check Valve : Intelligent Intake Valve
11) Maximum Operating Pressure : 15,000 psi up to 1mL/min, 9,000 psi up to 2 ml/min of better
12) Composition Ripple(baseline noise) : <1.0 mAu
13) Composition Accuracy : ±0.5%
14) Composition Precision : <0.2% RSD
15) Compressibility Compensation : Automatic and continuous
16) Flow Precision : 0.075% RSD
17) Flow Accuracy : ±1.0%
18) Priming : Wet priming can run at flow rates up to 4mL/min.
19) Flow Ramping Range : 0.01 to 30.0 min to reach 2.0mL/min
20) Primary Wetted Materials : 316L Stainless Steel, PPS, fluoropolymer, fluoroelastomer, UHMWPE, Sapphire, Ruby, Zirconia ceramic, Nitronic 60, DLC, PEEK and PEEK blend, Titanium alloy.
21) Unattended operation : Leak sensors, full 96-hour diagnostic data display through console software.
22) Number of Sample Plates : Total of two(2) plates (see below);
● 96 and 384 microtiter plates
● 48 position 2-mL Vial plates
● 48 position 0.65 mL micro-centrifuge tube plates
● 24 position 1.5 mL micro-centrifuge tube plates.
23) Injection Volume Range : 0.1~10.0 uL as standard. Up to 250.0uL
24) Accuracy (aspiration) : 10.0uL ± 0.2 uL
25) Precision : < 1% RSD 0.2 to 1.9 uL, < 0.5% RSD 2.0 to 10.0 uL, 6 replicates, 10:90 ACN/H2O, isocratic, 0.6 mL/min
26) Injector Linearity : > 0.999 (standard needle)
27) Sample Temperature Control : 4.0 to 40.0°C
28) Temperature Accuracy : +/- 0.5 °C
29) Temperature Stability : +/- 1.0 °C
30) Injection needle wash : Integral, active, programmable
31) Minimum Sample Required : 3 uL residual, Using total recovery 2-mL vials
32) Sample Carryover : <0.004% caffeine(UV), <0.005% sulphadimethoxine(MS)
33) Cycle time : < 30s inject-to-inject
34) Advanced Sample Manager Capabilities : Auto-dilution and transfer
35) Column Heater : Accommodates one column, up to 150 mm length x 4.6 mm ID in length with filter or guard column
36) Column Temperature Control : 20.0°C to 90.0°C, 0.1°C increments.
37) Column Tracking : Usage information tracked via Data Storage Device.
38) Temperature Stability : ±0.3°C
39) Temperature Accuracy : ±0.5°C
40) Solvent Conditioning : Active pre-heating, as standard. Passive pre-heating (also accommodated for legacy support)
41) Column Tracking : eCord Technology column information management tracks and archives column usage history.
42) Instrument Console
- System control, status monitoring, and user diagnostic capabilities are contained in the Instrument Console.
- Instrument functionality is accessible through this common instrument interface software.
- Instrument Console allows you to employ the integrated fluidics as part of Intellistart.
Additionally, they can be operated manually or programmed as part of an UPLC/MS method for flow divert or combined infusion.