Sony
SH800S
10년
주장비
분석
화합물 전처리·분석장비 > 바이오제조/분석장비 > 유세포분리/분석장치
2023-08-02
268,073,180원
없음
항체 개발 시 항체표적을 세포에 과발현시켜서 과발현된 세포의 분리하기 위해 필요하며, 항체 유효성 검증에 필수적인 장비
본 장비는 분석과 분류(sorting)의 기능을 모두 수행하며, 주로 분류기능에 특화된 장비로, Flow cytometry을 기반으로 한 기법으로서 적당한 형광물질로 표지되거나 특수한 형광을 지닌 세포를 가는 관을 통해 흘리면서 형광 유무 및 강도에 따라 분류 또는 계수하는 장치. 두 개의 채널을 통해 세포타입을 구분할 수 있으며, 분리된 세포의 분석 데이터 값을 역추적 가능.
기관의뢰
고정형
시간별
50,500원
본 장비는 분석과 분류(sorting)의 기능을 모두 수행하며, 주로 분류기능에 특화
- 두 개의 채널을 통해 세포타입을 구분할 수 있으며, 분리된 세포의 분석 데이터 값을 역추적 가능
- 유세포 분리기는 Flow cytometry을 기반으로 한 기법으로서 적당한 형광물질로 표지되거나 특수한 형광을 지닌 세포를 가는 관을 통해 흘리면서 형광 유무 및 강도에 따라 분류 또는 계수하는 장치
○ Excitation Laser Module: 488nm blue laser (30mW), 638nm red laser (30mW)
- Auto Power Control (APC) accuracy: ±2% for laser diodes ±3% for diode pumped solid-state (DPSS) laser
○ Detection Module
- Channels: 1x forward scatter(FSC), 1x back scatter(BSC), 6x fluorescence(FL)
- Analog-to-digital converters (ADC): 8-channel 20-bit effective resolution
- Light detectors: Photodiode (FSC), Photomultiplier tubes (BSC, FL)
- Fluorescence detection wavelength: 425 nm to 815 nm
- User exchangeable fluorescence optical filters
- Pulse measurement : Height, width, and area for all channels
- All measurements can be correlated against time parameter for kinetic analysis
- Threshold parameter: Single pulse threshold setting, selectable from all channels
○ Sorting chip
- The sorting chip automatically provides the system with nozzle size and optimal setting for stable reliable performance
- Replaceable sorting chip: The sorting chip, including the nozzle, can be easily replaced
- Nozzle size : 70μm, 100μm, 130μm
○ Sample Injection Chamber
- Sample loader: Single, auto-loading tube
- Sample input sizes: 1.5ml, 5.0ml and 15ml tubes or more
- Agitation unit: Eccentric rotation magnetic drive 300rpm speed
- Sample flow pressure: 50kPa to 300kPa (7 to 43 psi)
- Sample flow rate : 6μl/min (minimum)~168ul/min (maximum)
- Fluidics cleaning modes included in software: Automated startup, shut-down, Clean flow cell, Clean sample injection chamber
- Replaceable Sample Path: The sample path, including the sample line and the sample probe, can be easily exchanged
○ Cell Sorting Setup and Performance
- Two-way sorting into 5.0ml and 15.0ml tubes
- Plate sorting deposition : 6, 24, 48 and 96 well plates
- Sorting rate : Using the 70μm sorting chip at 50 kHz, an average threshold of 12,000 events per second can be achieved with >98% purity and >80% yield. A threshold rate up to 30,000 events per second can be achieved without affecting purity but with a decrease in yield based on Poisson’s statistics
- Sorting purity : More than 98%
- Sorting recovery : More than 80%
- Sorting viability : More than 90%
- Index sorting: Post sort analysis of multi-well plate data or equiv, Identify the sorted cells of each well on plots, statistics views and results file or equiv
- Automated calibration setup: optical axis adjustment, droplet calibration, side stream calibration, drop delay calibration
- Automated sort monitoring: software monitors and actively makes adjustments to the drop drive and side stream. It ensures consistently good sort performance and allows detection of clogs, empty tubes
○ Signal processing and system performance
- Fluorescence sensitivity: FITC ≤120 MESF, PE ≤1100 MESF
- Maximum event rate: 100,000 eps
- Fluorescence resolution: <3.0% coefficient of variation (CV): PI stained CEN
- Linearity : FITC R2 > 0.98, PE R2 > 0.98
- Automatic fluorescence compensation
- Analyzer mode: flow cytometry analysis can be performed without droplet and side stream calibration setup