ZEISS
LSM900
10년
주장비
시험
광학·전자 영상장비 > 현미경 > 공초점현미경
2022-12-23
374,199,050원
기관의뢰 직접사용
고정형
시간별
[Hr] 118,000원
Ⅰ. 용도(End-user’s Use)
1. Infinity color corrected optics with infinite image distance, it guarantees brilliant, aberration-
free images
2. Tile and multi-position with multi experimental capabilities.
3. Fast scanning with lowest bleaching capabilities: maintains homogeneous linearity during
scanning by constant monitoring and calibrating the scanner position.
4. Motorized Inverted microscope with software controlled peripherals
5. High sensitive GaAsP detectors for the highest signal to noise ratio imaging
6. True super resolution imaging using dedicated hardware unit
Ⅱ. 장비의 구성(Configurations of Goods)
1. 주장비(Main body)
- Scanner system 1 set
- Laser and electronic system 1 set
- System software 1 set
- Inverted microscopy system 1 set
2. 부속장비(Accessory)
- AVR: 3KV 1 ea
- Anti-vibration table: Air damped 1 ea
Ⅲ. 주장비 성능 및 규격(Performance and Specification)
1. Scanner System
1) Scanners
o 2 independent, galvanometric scan mirrors with constant monitoring and calibrating the
scanner position. It guarantees a stable field of view and equal pixel integration times over
the whole field of view
o Absolutely linearized scan movement to ensure equal pixel dwell-times as a prerequisite
for any quantitative study
2) Scan Zoom
o 1x to 40x: Digitally adjustable in 0.1 steps or wider
3) Scanning speed
o 8 frames/sec with 512x512 pixels or faster
4) Image scan
o Scan resolution: 4,096 x 4,096 pixels also for multiple channels; continuously adjustable (for
each axis) or better
o Any shapes of scanning format is possible, for example, round, star shape, etc.
o Scanning rotation: Free rotation (360°), in steps of 1° variable; free xy offset
5) High Dynamic Range
o 8-bit, 16-bit, or better 6) Pinholes
o Master-pinhole pre-adjusted in size and position
o Individually variable for multi-tracking and short
wavelengths
o Computer controlled automatic alignment
7) Detectors
o 2 sensitive GaAsP detectors
o Simultaneous 3 channels detection in confocal mode
o 1ea motorized transmitted-light PMT detector
2. Lasers & Electronic System
1) Lasers
o Diode laser (405nm) 5mW or better
o Diode laser (488nm) 10mW or better
o Diode laser (561nm) 10mW or better
o Diode laser (640nm) 5mW or better 2) Laser coupling unit: Laser inserts (VIS, V)
o Direct-coupled lasers with polarization preserving single-mode fibers
3) Electronics system
a) Control electronics
o Control of the microscope, the laser, scan module and other accessory components
o Control of the data acquisition and synchronization by real-time electronics
o Data communication between real-time electronics and user PC via Gigabit-Ethernet
interface with the possibility of online data analysis during image acquisition
b) Microscopy Workstation
o Premium Intel Xeon multilingua, Processor Intel Xeon Gold 5222, Memory 128 GB (4x32)
DDR4-2666 MHz ECC registered RAM, Graphic Card NVIDIA Quadro RTX4000 8GB mDP,
Hard Drive Extension 12TB RAID10
o 32 inch monitor
3. System Software
1) System configuration
o Comfortable configuration of all motorized the scanning, the lasers and the microscope
o Saving and restoring of application-specific configurations (ReUse)
o 64-Bit Operating system
2) System self-test
o Calibration and testing tool for the automatic verification and optimal adjustment of
the system
o Calibration objective for system calibration and adjustment
3) Acquisition modes, Smart setup
o Spot, line/spline, frame, z-stack, lambda stack, time series and all combinations
o Step scan (for higher frame rates)
o Smart acquisition setup by selection of dyes
4) Crop function
o Convenient and simultaneous selection of scanning areas (zoom, offset, rotation)
5) Real ROI scan, spline scan
o Scanning of up to 99 arbitrarily shaped ROIs (Region of Interest)
o Pixel precise switching of the laser
o ROI definition in z (volume)
o Scan along a freely defined line
6) ROI bleach
o Localized bleaching of up 99 bleach ROIs
o Use of different speeds for bleaching and image acquisition, use of different laser lines for
different ROIs
7) Image archiving, exporting & importing
o Functions for managing of images and respective recording parameters
o Multi-print function
o Over 20 file formats (TIF, BMP, JPG, PSD, DCX, GIF, AVI, Quicktime, etc) for export
8) Tiles & Positions - Mosaic scan for large surfaces (multiple XZ profiles and XYZ stacks) in
fluorescence & brightfield mode. Stitching algorithms perform in X, Y & Z-axis.
o Advanced mode: predefined sample areas, plus acquistion of images according to a position
list. Supports focus correction maps.
4. Inverted Microscopy System
1) Conventional mode: Brightfield, Phase-contrast, DIC, Fluorescence`
2) Laser mode: DIC, Fluorescence
3) Online overlay of transmitted-light Ph/DIC and confocal fluorescence
4) Conventional transmitted-light and reflected-light are overlaid at the same time
5) Motorized changing of conventional and laser scanning mode
6) Main body
o Exclusive microscope for laser scanning
o Motorized 6H nosepiece with automatic component recognition or more
7) Binocular tube
8) Illuminator: 12V 100W halogen lamp illuminator
o FL Filter set: Suitable for fluorescent dyes like DAPI, GFP, Rhodamin with excitation
wavelengths 405, 488, 561 and 640 nm.
9) Eyepieces
o The new observation eyepieces ideally complement the super quality of the Infinity
optics and optimally suited for all Infinity objectives
o Wide field of view 23mm
10) Motorized Scanning Stage
o XY travel range 127 mm x 83 mm, max speed 50 mm/s, resolution: 0.1um or better
11) Condenser system
o LD condenser, N.A.0.55
12) Objective: All objectives are corrected for infinite image distance
o Plan-Apo 5x/0.16 or 4x/0.2
o Plan-Apo 10x/0.45 or better
o Plan-Apo 20x/0.8 or 20x/0.75
o C-Apo 40x/1.2 Water or better
o Plan-Apo 63x/1.4, Oil or 60x/1.49
13) Focusing control
o The nosepiece is moved for focusing with the coarse/fine focusing control
o Smallest Z drive increment: 10nm or less
14) Reflection light filters & Fluorescence Illumination
o Apochromatic corrected beampath for reflected and fluorescence illumination
o Motorized 6 position reflector revolver for Push & Click filter cubes with automatic
component recognition for ease of use
o Illuminator: 12V 100W halogen lamp illuminator
15) TFT Control Monitor
o Touch screen display
o Control full function of microscope operation
o Personal user settings
5. Super Resolution Unit 1) Set-up of the optical path is fully motorized and configured using the software
2) Laser line flexibility from 405nm to 639nm
3) Utilize a dedicated hardware method
4) 2 modes of operation: Super resolution, Confocal mode
5) Lateral resolution of 115nm or better
6) Axial resolution of 270nm or better
7) Image acquisition speeds o 512 x 512 pixels – 8 FPS or faster
o Suitable with z-stack, time series and tile images
Ⅲ. 주장비 성능 및 규격(Performance and Specification)
1. Scanner System
1) Scanners
o 2 independent, galvanometric scan mirrors with constant monitoring and calibrating the
scanner position. It guarantees a stable field of view and equal pixel integration times over
the whole field of view
o Absolutely linearized scan movement to ensure equal pixel dwell-times as a prerequisite
for any quantitative study
2) Scan Zoom
o 1x to 40x: Digitally adjustable in 0.1 steps or wider
3) Scanning speed
o 8 frames/sec with 512x512 pixels or faster
4) Image scan
o Scan resolution: 4,096 x 4,096 pixels also for multiple channels; continuously adjustable (for
each axis) or better
o Any shapes of scanning format is possible, for example, round, star shape, etc.
o Scanning rotation: Free rotation (360°), in steps of 1° variable; free xy offset
5) High Dynamic Range
o 8-bit, 16-bit, or better 6) Pinholes
o Master-pinhole pre-adjusted in size and position
o Individually variable for multi-tracking and short
wavelengths
o Computer controlled automatic alignment
7) Detectors
o 2 sensitive GaAsP detectors
o Simultaneous 3 channels detection in confocal mode
o 1ea motorized transmitted-light PMT detector
2. Lasers & Electronic System
1) Lasers
o Diode laser (405nm) 5mW or better
o Diode laser (488nm) 10mW or better
o Diode laser (561nm) 10mW or better
o Diode laser (640nm) 5mW or better 2) Laser coupling unit: Laser inserts (VIS, V)
o Direct-coupled lasers with polarization preserving single-mode fibers
3) Electronics system
a) Control electronics
o Control of the microscope, the laser, scan module and other accessory components
o Control of the data acquisition and synchronization by real-time electronics
o Data communication between real-time electronics and user PC via Gigabit-Ethernet
interface with the possibility of online data analysis during image acquisition
b) Microscopy Workstation
o Premium Intel Xeon multilingua, Processor Intel Xeon Gold 5222, Memory 128 GB (4x32)
DDR4-2666 MHz ECC registered RAM, Graphic Card NVIDIA Quadro RTX4000 8GB mDP,
Hard Drive Extension 12TB RAID10
o 32 inch monitor
3. System Software
1) System configuration
o Comfortable configuration of all motorized the scanning, the lasers and the microscope
o Saving and restoring of application-specific configurations (ReUse)
o 64-Bit Operating system
2) System self-test
o Calibration and testing tool for the automatic verification and optimal adjustment of
the system
o Calibration objective for system calibration and adjustment
3) Acquisition modes, Smart setup
o Spot, line/spline, frame, z-stack, lambda stack, time series and all combinations
o Step scan (for higher frame rates)
o Smart acquisition setup by selection of dyes
4) Crop function
o Convenient and simultaneous selection of scanning areas (zoom, offset, rotation)
5) Real ROI scan, spline scan
o Scanning of up to 99 arbitrarily shaped ROIs (Region of Interest)
o Pixel precise switching of the laser
o ROI definition in z (volume)
o Scan along a freely defined line
6) ROI bleach
o Localized bleaching of up 99 bleach ROIs
o Use of different speeds for bleaching and image acquisition, use of different laser lines for
different ROIs
7) Image archiving, exporting & importing
o Functions for managing of images and respective recording parameters
o Multi-print function
o Over 20 file formats (TIF, BMP, JPG, PSD, DCX, GIF, AVI, Quicktime, etc) for export
8) Tiles & Positions - Mosaic scan for large surfaces (multiple XZ profiles and XYZ stacks) in
fluorescence & brightfield mode. Stitching algorithms perform in X, Y & Z-axis.
o Advanced mode: predefined sample areas, plus acquistion of images according to a position
list. Supports focus correction maps.
4. Inverted Microscopy System
1) Conventional mode: Brightfield, Phase-contrast, DIC, Fluorescence`
2) Laser mode: DIC, Fluorescence
3) Online overlay of transmitted-light Ph/DIC and confocal fluorescence
4) Conventional transmitted-light and reflected-light are overlaid at the same time
5) Motorized changing of conventional and laser scanning mode
6) Main body
o Exclusive microscope for laser scanning
o Motorized 6H nosepiece with automatic component recognition or more
7) Binocular tube
8) Illuminator: 12V 100W halogen lamp illuminator
o FL Filter set: Suitable for fluorescent dyes like DAPI, GFP, Rhodamin with excitation
wavelengths 405, 488, 561 and 640 nm.
9) Eyepieces
o The new observation eyepieces ideally complement the super quality of the Infinity
optics and optimally suited for all Infinity objectives
o Wide field of view 23mm
10) Motorized Scanning Stage
o XY travel range 127 mm x 83 mm, max speed 50 mm/s, resolution: 0.1um or better
11) Condenser system
o LD condenser, N.A.0.55
12) Objective: All objectives are corrected for infinite image distance
o Plan-Apo 5x/0.16 or similar
o Plan-Apo 10x/0.45 or similar
o Plan-Apo 20x/0.8 or similar
o C-Apo 40x/1.2 Water or similar
o Plan-Apo 63x/1.4, Oil or similar
13) Focusing control
o The nosepiece is moved for focusing with the coarse/fine focusing control
o Smallest Z drive increment: 10nm or less
14) Reflection light filters & Fluorescence Illumination
o Apochromatic corrected beampath for reflected and fluorescence illumination
o Motorized 6 position reflector revolver for Push & Click filter cubes with automatic
component recognition for ease of use
o Illuminator: 12V 100W halogen lamp illuminator
15) TFT Control Monitor
o Touch screen display
o Control full function of microscope operation
o Personal user settings
5. Super Resolution Unit 1) Set-up of the optical path is fully motorized and configured using the software
2) Laser line flexibility from 405nm to 639nm
3) Utilize a dedicated hardware method
4) 2 modes of operation: Super resolution, Confocal mode
5) Lateral resolution of 115nm or better
6) Axial resolution of 270nm or better
7) Image acquisition speeds o 512 x 512 pixels – 8 FPS or faster
o Suitable with z-stack, time series and tile images