GE Healthcare
AKTA Avant 150
10년
주장비
분석
화합물 전처리·분석장비 > 분리분석장비 > 분취용크로마토그래피
2020-11-23
124,821,000원
기관의뢰 직접사용
고정형
시간별
100,000원
- 미생물배양기반 백신의 생산 공정개발을 위해 다양한 단백질, 핵산 등 백신 원료물질의 분석 및 분리 정제 조건을 탐색하기 위한 목적으로 사용
- 목표 물질 (단백질, 핵산 등 생물 관련 고분자물질과 저분자 물질)의 크기, 이온성 혹은 특이성 등에 따라 다양한 resin을 사용함으로써 효과적인 크로마토그래피 공정 확립에 이용
○ Small scale(Avant)
1) System pump (Piston pump)
- Flow rate : 0.01 ~ 150ml/min (Normal range)
0.01 ~ 300ml/min (Column packing flow)
- Pressure range : 0 ~ 5MPa (0 ~ 725psi)
- Viscosity range : 0.35 ~ 5.0cP
- Flow rate specifications : Accuracy ± 1.5%
Precision : RSD < 0.5% (1 ~ 150ml/min, < 3MPa,
0.8 ~ 2cP)
2) Sample pump (Piston pump)
- Flow rate : 0.01 ~ 150ml/min
- Pressure range : 0 ~ 5MPa (0 ~ 725psi)
- Viscosity range : 0.7 ~ 10cP
- Flow rate specifications : Accuracy ± 2%
Precision : RSD < 0.5% (1 ~ 150ml/min, < 3MPa, 0.8 ~ 3cP)
3) Gradient mixer (Chamber with magnetic stirrer)
- Mixer volume : 1.4, 5(Default), 15ml
- Gradient flow rate range : 1 ~ 150ml/min (Binary)
- Gradient composition accuracy : ± 0.6% (Binary), ± 1.0% (Quaternary)
(Conditions : 5 ~ 95% B, 2 ~ 150ml/min, 0.2 ~ 2MPa, 0.8 ~ 2cP)
4) Valve (Rotary type)
- Buffer inlet valve : 7 Positions, expandable to 14
- Sample inlet valve : 7 Positions, expandable to 14
- Injection valve
⸱ Sample loading : Fixed sample loops, sample loading pump
- Column valve : Connects up to 5 columns, column switching, bypass, reverse flow
- Outlet valve : 10 Positions, expandable to 32
5) UV and UV/Vis detectors
- Multi-wavelength : 190 ~ 700nm in steps of 1nm, up to 3 wavelengths simultaneously
- Absorbance range : -6 ~ 6Au
- Linearity : 0 ~ 2Au within ±2%
- Drift : ≤0.2mAu(2mm cell at 280nm)
- Noise : <0.08mAu
6) Pressure sensor
- Number of sensors : 4 (After system pump, after sample pump,
pre-column valve, post-column valve)
- Pressure reading range : 0 ~ 5MPa(0 ~ 725psi)
- Accuracy : ±0.015MPa
7) Conductivity monitor
- Conductivity range : 0.01 ~ 999.99mS/cm
- Accuracy : ±2%
- Operating pressure : 0 ~ 5MPa(0 ~ 725psi)
- Flow cell volume : 22μl
- Temperature reading range : 0 ~ 99℃
- Temperature reading accuracy : ±1.5% within 4 ~ 45℃
8) pH monitor
- pH reading range : 0 ~ 14
- Accuracy : ±0.1 pH unit
- Operating pressure : 0 ~ 0.5MPa(0 ~ 73psi)
- Flow cell volume : 129μl
9) Fraction collector
- Rack type : Well plate, tube, bottle
⸱ 96, 48, 24 well plate
⸱ 3, 8, 15, 50ml tubes
⸱ 250ml bottle
- Fraction volume : 0.1 ~ 250ml
- Delay volume : 1807㎕
10) System software : Unicorn 7 Workstation (Include of DoE program)
- 21CFR11 compliance