Molecular Devices
SPECTRAMAX ID5
10년
주장비
분석
화합물전처리/분석장비 > 분광분석장비 > 형광분광광도계
공동활용허용
2018-06-01
101,863,730원
기관의뢰
고정형
건별
21,000원
- 형광, 흡광, 발광 및 TRF, FP, FRET, TR-FRET 등의 측정이 가능.
- 측정과정에서 어떠한 파장도 임의로 선택이 가능함.
- 미지의 샘플에 대한 파장 스캐닝이 가능.
- 65℃까지 온도조절이 가능하며, 6 ~ 384 wells를 사용하여 샘플을 측정할 수 있음
- 2 Channel injector를 통하여, 각각의 well에 원하는 볼륨, 속도로 분주가 가능함.
1. Main Body
1) Detection Mode
(1) Detection - Absorbance, Fluorescence, & Luminescence
TRF, Fluorescent Polarization (tunable), TR-FRET (HTRF),
Western blot with ScanLater Western Blot kit,
Dual color luminescence (BRET)
Superior IR (red-shifted) detection with specialized filters
Optional bottom read luminescence
(2) Spectral scanning : Absorbance, Fluorescence, & Luminescence, FP (1.0 nm increments)
(3) Well scanning - Over 20 by 20 in all modes or more
2) General instrument
(1) Robot compatible : Yes
(2) Compatibility
- Microplate type : 6 ~ 384-well microplates or wider
- Cuvette : via adapter or equivalent
- Low volume DNA detection tool : Spectradrop or equivalent
(3) Light source : Xenon Flash Lamp
3) Temperature regulation
(1) Range : 5°C above ambient to 66°C
(2) Accuracy : ± 1°C at 37°C set point
(3) Temperature uniformity at equilibrium : ±0.75 ℃
4) Autocalibrate : Automatic calibration before first kinetic read and before every end-point reading
5) Absorbance photometric performance
(1) Measurement : Optical Density (OD) or % Transmittance
(2) Measurement modes : Endpoint, Kinetic, Spectral scanning & Well scanning
(3) Light Source : Xenon Flash Lamp
(4) Optics : Fiber optics
(5) Photodetectors : Photodiode
(6) Wavelength range : 230 to 1000 nm or wider
(7) Wavelength selection Type : Monochromator or equivalent
(8) Wavelength selection : Monochromator tunable 1nm increments or less
(9) Wavelength bandwidth : ≤ 4nm FWHM(full width half maximum) or less
(10) Wavelength accuracy : < ±2.0nm across wavelength range or less
(11) Wavelength repeatability : ± 1.0 nm across all optical channels or less
(12) Photometric range : 0 to 4.0 OD or wider
(13) Photometric resolution : 0.001 OD or less
(14) Photometric accuracy : < ±0.010 OD and ± 1% , 0-3.0 OD or less
(15) Photometric precision : < ±0.003 OD and ± 1% , 0-3.0 OD or less
6) Fluorescence photometric performance
(1) Measurement modes : Endpoint, Kinetic, Spectral scanning & Well scanning,
(2) Light Source : Xenon flash lamp
(3) Detector : Ultra-cooled PMT or equivalent
(4) Reading capability : Top-reading & Bottom-reading
(5) Wavelength range ( monochromator ) :
① Excitation : 250 - 830nm ( monochromator ) or wider
② Emission : 270 - 850nm ( monochromator ) or wider
(6) Wavelength range ( filters ) : can select filters in accessory list
(7) Wavelength selection : Scanning monochromator tunable in 1 nm increments or less
and can select filters in accessory list
(8) Fluorescence sensitivity
① Microplate(Top read)
- 1 pM /well, 96 wells ( monochromator ) or less
- 1 pM /well, 384 wells ( monochromator ) or less
- 0.3 pM /well, 96 wells ( filter) or less
- 0.5 pM /well, 384 wells ( filter ) or less
② Microplate(Bottom read)
- 2 pM /well, 96 wells ( monochromator ) or less
- 2 pM /well, 384 wells ( monochromator ) or less
- 2.5 pM /well, 96 wells ( filter) or less
- 2.5 pM /well, 384 wells ( filter ) or less
7) Luminescence photometric performance
(1) Detector : Ultra-cooled PMT for luminescence or equivalent
(2) Wavelength range : 300 – 850nm or wider
300 - 650 nm for all wavelength setting
(3) Crosstalk : < 0.1% in 96-well and < 0.2% 384-well microplates or less
(4) Luminescence sensitivity : 20 amol ATP in 96-well or less
8) Time-Resolved Fluorescence performance
(1) Detector : Ultra-cooled PMT or equivalent
(2) Wavelength range : 450 - 750nm (EM mono) or wider
350 nm (EX filter)
490 nm (Terbium), 616 nm (Europium)
For other filters, please see accessory list ( EM filter)
(3) Linear dynamic range : Up to 5 logs or more
(4) Detection limit : Standard TRF
30 fM Europium (6 amol/well) ( 96 wells (white) ) or less
30 fM Europium (3 amol/well) ( 384 wells (white) ) or less
Enhanced TRF Module (Optional)
10 fM Europium (2 amol/well) ( 96 wells (white) ) or less
10 fM Europium (1 amol/well) ( 384 wells (white) ) or less
9) Fluorescence polarization performance
(1) Detector : Ultra-cooled PMT or equivalent
(2) Wavelength range : (300-) 400–750 nm, 1.0 nm increments ( (EX mono) or wider
(300-) 400–750 nm, 1.0 nm increments (EM mono) or wider
Wavelength range (Filters) - can select filters in accessory list
(3) Detection limit : 2 mP @ 10 nM ( 96 wells black - Monochromator ) or less
1 mP @ 1 nM ( 96 wells black - Filtersr ) or less
2 mP @ 10 nM ( 384 wells black - Monochromator ) or less
2 mP @ 10 nM ( 384 wells black - Filtersr ) or less
(4) Measurement range : Delta > 200 mP ( Monochromator ) or less
Delta > 320 mP ( Filtersr ) or less
10) Photometric analysis modes
(1) Using Data aquisition and analysis program
① Express data as Absorbance, % Transmittance, fluorescence, luminescence
② Single wavelength reading of microplate and/or cuvette
③ Multiple wavelength reading of microplate and/or cuvette
④ Kinetic and kinetic graphics of microplate and/or cuvette
⑤ Spectral scan of microplate and/or cuvette
⑥ Well scan of microplate using absorbance, fluorescence intensity, luminescence & TRF
11) Measurement time
(1) Microplate
① Read time : 10 msec integration time for fluoresence measurement. or less
100 msec integration time for luminescence for a 96-well plate
and 40 msec integration time for a 384-well plate. or less
- 96 wells in 30 seconds(single wavelength, absorbance) or less
- 384 wells in 1min 30 seconds (single wavelength, absorbance) or less
- 96 wells in 30 seconds (single wavelength, fluorescence) or less
- 384 wells in 1 min 30 seconds (single wavelength, fluorescence) or less
- 96 wells in 30 seconds (single wavelength, luminescence) or less
- 384 wells in 1min 30 seconds (single wavelength, luminescence) or less
- 96 wells in 30 seconds (single wavelength, Time-Resolved Fluorescence) or less
- 384 wells in 1min 30 seconds (single wavelength, Time-Resolved Fluorescence) or less
- 96 wells in 1 mins (single wavelength, Fluorescence Polarization) or less
- 384 wells in 3 min (single wavelength, Fluorescence Polarization) or less