Molecular Device
Spectramax I3
10년
주장비
분석
화합물 전처리·분석장비 > 분광분석장비 > 형광분광광도계
2018-04-10
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흡광(Absorbance), 형광(Fluorescence), 발광(Luminescence)과 같은 광학적 성질을 갖는 물질 및 기질을 이용한 실험을 통해서 상호 연관성 및 차이를 쉽게 분석할 수 있는 장치로 microplate를 이용하여 다량의 시료를 한번에 측정 분석 가능한 학술연구용 장비임. Cell-based assay, Marker expression assay, Luciferase assay 등의 다양한 실험 수행할 수 있으며, 근적외선 영역의 파장 측정을 통해 in vitro 수준에서 비임상 효력시험용 장비임
1) Detection Mode
① Detection
- Absorbance, Fluorescence, Luminescence, TR-F(FRET), FP, AlphaScreen/AlphaLISA
② Spectral scanning :
- Absorbance, Fluorescence
- Luminescence (1.0 nm increments) or less
③ Well scanning
2) General instrument
① Robot compatible
② Compatibility
- Microplate type: (6, 12, 24, 48, 96, 384-well, 1536 well) or more
③ Light source
- Spectral Fusion Illumination (Xenon flash lamp or more)
④ Shaking : Linear and Orbital
3) Absorbance photometric performance
① Measurement
- Optical Density (OD) or Transmittance(%)
② Measurement modes
- Endpoint, Kinetic, Spectral scanning & Well scanning
③ Light Source : Xenon Flash Lamp
④ Optics : Fiber optics
⑤ Photodetectors : Photodiode
⑥ Wavelength range : 230 to 1000 nm or wider
⑦ Wavelength selection Type :
- Monochromator or filter(upgradable with monochromator)
⑧ Wavelength selection
- Monochromator tunable 1nm increments or less
⑨ Wavelength bandwidth
: ≤ 8nm FWHM(full width half maximum)
⑩ Wavelength accuracy
: ≤ ±2.0nm across wavelength range
⑪ Wavelength repeatability
: ± 1.0 nm across all optical channels or less
⑫ Photometric range : 0 to 4.0 OD or higher
⑬ Photometric resolution : 0.001 OD or less
4) Fluorescence photometric performance
① Measurement modes
- Endpoint, Kinetic, Spectral scanning & Well scanning
② Light Source
- Spectral Fusion Illumination (Xenon flash lamp or more)
③ Detector : High QE Multialkali Photocathode PhotoMultiplier Tube(PMT) for fluorescence
④ Reading capability
- Top-reading & Bottom-reading
⑤ Wavelength range
: Excitation : 250 - 850nm or wider
: Emission : 250 - 850nm or wider
⑥ Wavelength selection
: Scanning monochromator tunable in 1 nm increments or less
5) Luminescence photometric performance
① Detector
- High sensitivity Photo Multiplier Tube(PMT) for luminescence
② Wavelength range : 300 – 850nm
③ Luminescence sensitivity(signal 3X standard deviation of baseline)
: 15 pM/well, ATP at 96-well or better
: 30 pM/well, ATP at 384-well or better
6) Temperature regulation
① Range : 4 ℃ above ambient to 45 ℃ or higher
② Accuracy : ±1.0 ℃ at 37 ℃ set point
③ Temperature uniformity at equilibrium : ±1.0 ℃ or less