Bio-Rad
Biolistic PDS-1000/He
10년
주장비
시험
기타 > >
2009-10-22
54,702,440원
고정형
시간별
4,600원
1. This system has capable of accelerating sub-cellular sized tungsten or gold microprojectiles coated with DNA over a range of velocities and target distances necessary to optimally transform many different cell types while conventional transformation system has limited application range of target samples. 2. This system is a convenient method for transforming intact cells in culture since very little pre-or-post-bombardment manipulation such as chemical treatment is necessary and sample containment within the evacuated bombardment chamber offers maximum control. 3. It allows transformation of animal cells with unique growth requirements that are not amenable to gene transfer using electroporation or chemical transfection. 4. Transform cultured mammalian cells with the device shows more than 30% improved transient transformation of variety of sensitive mammalian cells that are poorly transformed with chemicals lipofection and even electroporation. 5. It can use less DNA(~200ng) and fewer cells(108~109 per 100mm plate) for either transient or stable transformation with the system. 6. This technique processes transfection within 30 min from pre-preparation to DNA introduction and much easier task compared to microinjecting embryos.
B. System Configulation : 1. Bombardment chamber and Control panel 2. Microcarrier Launch Assembly 3. 3-way Helium Metering(Solenoid) valve _x000D_
1. Bombardment chamber and Control panel - Launch velocity of microcarriers depended on adjustable parameters 1) Helium pressure to burst Rupture Disk : 450-2200 psi 2) amount of vacuum adjustable by control valve: 10-29 Hg 3) the distance from the rupture disk on the macrocarrier: 3 6 9 cm 4) the distance from the microcarreier launch assembly to the stopping screen