∙ The LumiCycle apparatus fits inside a standard incubator. An internal fan circulates the incubator air to
maintain the proper temperature within the chamber. The temperature is therefore as stable as the incubator
can make it. The turntable and photon counting are fully automated.
∙ Two-color option. With the 2-color adaptation, colored filters can be placed on 2 of the photomultipliers so that 16 samples can be recorded simultaneously in 2 different colors. To create the image on the right, fibroblasts were transfected with Bmal-Nluc and Per2-Fluc, and recorded with a 550 nm long-pass filter on one PMT and 500 nm short-pass filter on the other. Optical filters can be easily changed by the user, or they can be removed for recording in the standard, non-color mode.