1.Automatic Measurement of Ploidy Level and Aneuploidy in Plant _x000D_
Ploidy measurement in less than two minutes_x000D_
Any plant material can be analysed: leaves seedlings roots flowers peel of fruits seeds Preparation and staining of metaphase chromosomes is not necessary; no time-consuming _x000D_
microscopic evaluation; no chromosome counting_x000D_
Detection of aneuploidy with +/- 1 chromosome accuracy in most plants 2.Measuring principle _x000D_
Ploidy determinations with the Partec PA Ploidy Analyser are performed by the precise _x000D_
measurement of the total DNA content of individual nuclei which can be derived from almost _x000D_
any plant tissue. The Ploidy Analyser measures the DNA content of many thousands of individual nuclei _x000D_
within seconds or minutes. The result is displayed as a DNA histogram in real time on a large LCD screen together with the ploidy level. ploidy analysis is extremely time and cost efficient. 3.Procedure for fresh or frozen plant material _x000D_
approx 0.5 cm square leaf or other plant material _x000D_
chop with a sharp razor blade in 0.5 ml of Partecsolution A in a plastic petri dish _x000D_
filter the sample through a 30 ？m partec CellTrics directly into the sample tube _x000D_
wait 1 to 15 min at room temperature (best time must be determined for each plant variety) _x000D_
add at least 2 ml Partec CyStain UV solution B _x000D_
connect sample tube to the Ploidy Analyser wait approx. 20 sec for optimal DNA histogram solution B