1. Fully Protein Prep software supported autotuning facilities including ; Automatic calibration routines, Prep inlet method, API (ESI in positive and negative ion modes) MS analysis, Data-base search, Peptide de-novo sequencing, Protein identification and Metabolite ID. 2. The Nano spray system is acquired high quality Protein Prep data from limited sample amounts as well as ultimate in resolution, sensitivity and speed or require a simple, easy-to-use, direct nano-flow solution with unparalleled retention time reproducibility.